TORC3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human TORC3 Monoclonal Antibody (Catalog # MAB9455) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TORC3 Antibody

Application

Recommended Usage

Immunocytochemistry

1-10 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TORC3

CREB-Regulated Transcription Coactivator 3 (CRTC3), also known as TORC3, a transcriptional coactivator for CREB1, acts as a coactivator in the SIK/TORC signaling pathway. Dephosphorylated CRTC3 increases the interaction of CREB1 cAMP response element (CRE) sites. CRTC3 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its binding to the HTLV-1 protein Tax enhances HTLV-1 transcription, whereas its binding to cellular Bcl3 inhibits HTLV-1 transcription. CRTC3 polymorphism have been associated with obesity and hypertriglyceridemia.

Long Name

Transducer of Regulated CREB-binding Protein 3

Alternate Names

CRTC3

Entrez Gene IDs

64784 (Human); 70461 (Mouse); 365297 (Rat)

Gene Symbol

CRTC3

UniProt

Q6UUV7

Additional TORC3 Products

Product Documents for Human TORC3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TORC3 Antibody

For research use only

Citations
Reviews

Human TORC3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9455

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TORC3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TORC3 Antibody

TORC3 in HeLa Human Cell Line.