Detects human TCF-3/E2A in direct ELISAs. In this format, 100% cross-reactivity with recombinant human (rh) TCF-12 is observed and no cross-reactivity with rhTCF-1, 2, 4, 7L1, 7L2 or rhLEF-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human TCF-3/E2A Antibody

TCF-3/E2A in HeLa Human Cell Line.

TCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TCF-3/E2A Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF-3/E2A

TCF-3, also known as E2A and ITF-1, is a member of the E protein homeobox family. TCF-3 regulates checkpoints that allow B lineage commitment and B and T cell receptor gene rearrangement. Two isoforms named E12 and E47 are alternatively spliced within the DNA binding and HLH domain. The region of human TCF-3 used as the immunogen, which is identical for E12 and E47, shares 80% and 79% aa identity with mouse and rat TCF-3, respectively.

Long Name

Transcription Factor 3/E2-alpha

Alternate Names

bHLHb21, E2A, ITF1, Kappa-E2-binding factor, Pan2, Tcfe2a, VDIR

Entrez Gene IDs

6929 (Human); 21423 (Mouse); 312451 (Rat)

Gene Symbol

TCF3

UniProt

NP_003191

Additional TCF-3/E2A Products

Product Documents for Human TCF-3/E2A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human TCF-3/E2A Antibody

For research use only

Citations
Reviews

Human TCF-3/E2A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB44061

Key Product Details

Species Reactivity

Validated:

Cited:

Applications