Jurkat cells were stained with Mouse Anti-Human Survivin PE-conjugated Monoclonal Antibody (Catalog # IC6472P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Survivin PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) family and can inhibit apoptosis induced by a variety of factors. It is expressed in most human cancers but not in normal adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human)

Gene Symbol

BIRC5

UniProt

O15392

Additional Survivin Products

Product Documents for Human Survivin PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Survivin PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Survivin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC6472P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Survivin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Survivin PE-conjugated Antibody

Detection of Survivin in Jurkat cells by Flow Cytometry