SUMO-Specific Peptidase 1/SENP1 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human SUMO-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SUMO1-Specific Peptidase 1/SENP1 in Human Testis.

SUMO1-Specific Peptidase 1/SENP1 was detected in immersion fixed paraffin-embedded sections of human testis using Sheep Anti-Human SUMO1-Specific Peptidase 1/SENP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6587) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to late spermatids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SUMO1-Specific Peptidase 1/SENP1 Antibody

Application

Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed T47D human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SENP1

SENP1 (Sentrin/SUMO-specific protease) is a member of the SENP family of proteases. Sentrin/SUMO-specific proteases (SENPs) are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length SUMOs to their mature forms and deconjugation of SUMOs from SUMOlyated proteins. The seven mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity. Human SENP-1 has broad specificity for the three mammalian SUMOs. It is found in the cytoplasm and nucleus depending on cell type, and is expressed in testis, thymus, pancreas, spleen, liver, ovary and small intestine. It is thought that localization of SENP-1 is vital for the regulation for SUMOylation status of target proteins. The small ubiquitin-like modifier (SUMO) is a member of ubiquitin-like protein family. SUMO modification of the target proteins is a reversible process that regulates many cellular processes including transcription regulation, nuclear localization, centromere segregation and signal transduction. The recombinant human SENP-1 (immunogen) includes the catalytic domain, which has been shown to be sufficient for SENP-1 activity and substrate specificity.

Long Name

SUMO-Specific Peptidase 1

Alternate Names

SuPr-2

Entrez Gene IDs

29843 (Human); 223870 (Mouse); 300193 (Rat)

Gene Symbol

SENP1

UniProt

Q9P0U3 (Human)

Additional SENP1 Products

Product Documents for Human SUMO1-Specific Peptidase 1/SENP1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human SUMO1-Specific Peptidase 1/SENP1 Antibody

For research use only

Citations
Reviews

Human SUMO1-Specific Peptidase 1/SENP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6587

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SENP1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SUMO1-Specific Peptidase 1/SENP1 Antibody

SUMO‑Specific Peptidase 1/SENP1 in T47D Human Cell Line.