Human Sulfatase Modifying Factor 2/
SUMF2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2779
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 2/SUMF2
Gln26-Leu301
Accession # Q8NBJ7
Gln26-Leu301
Accession # Q8NBJ7
Specificity
Detects human Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant mouse SUMF2 is observed and less than 1% cross-reactivity with recombinant human SUMF1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Sulfatase Modifying Factor 2/SUMF2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Sulfatase Modifying Factor 2/SUMF2, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Sulfatase Modifying Factor 2/SUMF2, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Sulfatase Modifying Factor 2/SUMF2
Sample: Recombinant Human Sulfatase Modifying Factor 2/SUMF2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sulfatase Modifying Factor 2/SUMF2
Mammalian sulfatases have a common CXPXR motif, in which the Cys residue is post-translationally modified to a Calpha-formylglycine (FGly) residue. This modification is essential for catalytic activity of all sulfatases and is catalyzed by FGly generating enzyme (FGE) encoded by sulfatase modifying factor 1 (SUMF1). FGE deficiency causes multiple sulfatase deficiency (MSD), a rare autosomal recessive disorder. SUMF2 does not have FGly enzymatic activity, but may compete with SUMF1 for the same substrate.
Additional Sulfatase Modifying Factor 2/SUMF2 Products
Product Documents for Human Sulfatase Modifying Factor 2/SUMF2 Antibody
Product Specific Notices for Human Sulfatase Modifying Factor 2/SUMF2 Antibody
For research use only
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