SLX4 was detected in immersion fixed RAW 264.7 Mouse Monocyte/Macrophage Cell line (Mouse) and A431 Human Epithelial Carcinoma Cell Line (Human) Cells using Rabbit Anti-Human SLX4 Monoclonal Antibody (Catalog # MAB11246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human SLX4 Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed RAW 264.7 Mouse Monocyte/Macrophage Cell line (Mouse) and A431 Human Epithelial Carcinoma Cell Line (Human) Cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLX4

SLX4 (also known as BTBD12 and FANCP) is a multidomain scaffold protein involved in DNA repair, where it has important roles in the final steps of homologous recombination. SLX4 hat interacts with three distinct nucleases: SLX1, ERCC4/XPF-ERCC1 and MUS81-EME1. Mutations in the gene are associated with the disease Fanconi anemia, a rare recessive disorder characterized by genome instability, congenital malformations, progressive bone marrow failure and predisposition to hematologic malignancies and solid tumors.

Long Name

SLX4 Structure-Specific Endonuclease Subunit

Alternate Names

BTBD12, FANCP, KIAA1784, KIAA1987

Entrez Gene IDs

84464 (Human); 52864 (Mouse)

Gene Symbol

SLX4

Additional SLX4 Products

Product Documents for Human SLX4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLX4 Antibody

For research use only

Citations
Reviews

Human SLX4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11246

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all SLX4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human SLX4 Antibody

Detection of SLX4 in RAW264.7 (Mouse) and A431 (Human) cells .