Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all Serpin A12 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A12
Leu20-Lys414
Accession # Q8IW75

Specificity

Detects human Serpin A12 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin F1, and recombinant mouse Serpin E1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Serpin A12 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin A12

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A#12; also known as vaspin) is a 45‑50 kDa secreted member of the alpha1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral adipocytes, and adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown. Human Serpin A12 is 414 amino acids (aa) in length. It contains a 20 aa signal sequence and a 394 aa mature region. A potential "active" inhibitor site lies between Arg363 and Met378. Mature human Serpin A12 shares 61% aa identity with mouse Serpin A12.

Alternate Names

OL-64, Vaspin

Entrez Gene IDs

145264 (Human); 68054 (Mouse); 191570 (Rat)

Gene Symbol

SERPINA12

UniProt

Q8IW75

Additional Serpin A12 Products

Product Documents for Human Serpin A12 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin A12 Biotinylated Antibody

For research use only

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