Human RIG-I Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4859R
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RIG-I
Met724-Lys925
Accession # O95786
Met724-Lys925
Accession # O95786
Specificity
Detects human RIG-I in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human RIG-I Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: RIG-I
RIG-I (Retinoic-acid inducible gene I; also Dead-box protein 58) is a 100 kDa member of the DexH-box family of proteins. It is expressed in smooth muscle cells and endothelium. RIG-I is normally an inactive monomer. Upon Sendai and Hepatitis C virus infection, RIG-I binds to dsRNA, initiating homodimerization and downstream activation of IRF3 and KF kappaB genes. Human RIG-I is 925 amino acids (aa) in length. It contains two CARD domains (aa 1‑172), one helicase ATP-binding region (aa 251‑430), and one C-terminal repressor domain (aa 735‑925). There are three potential splice variants. One shows an alternate start site at Met204, while another shows deletion of aa 3‑80, and a third shows a deletion of aa 36‑80 plus 749‑925. Over aa 724‑925, human RIG-I is 76% aa identical to mouse RIG-I.
Long Name
RIG-I
Alternate Names
DDX58, RIGI
Gene Symbol
DDX58
UniProt
Additional RIG-I Products
Product Specific Notices for Human RIG-I Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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