Human/Rat Phospho-TrkA (Y785) Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5479AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human TrkA Y785 site
Specificity
Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: TrkA
The receptor tyrosine kinase TrkA binds nerve growth factor (NGF) with low affinity, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires a complex formed between TrkA and the p75 NGF receptor. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues, including Y490, Y751 and Y785. Phosphorylation of TrkA at Y785 creates a binding site for phospholipase C-gamma (PLC-gamma), which catalyzes the cleavage of substrate PIP2 to generate the second messengers DAG and IP3.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Alternate Names
NTRK-1, NTRK1
Gene Symbol
NTRK1
Additional TrkA Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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