Human blood-derived monocytes were stained with Human Prokineticin R1/PKR1 Monoclonal Antibody (Catalog # MAB4655, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B).

Applications for Human Prokineticin R1/PKR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human blood-derived monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prokineticin R1/PROKR1

Prokineticin Receptor 1 (PKR1), also called ZAQ or GPR73a (G-protein coupled receptor 73a), is a 7-transmembrane glycoprotein of the GPCR family. The extracellular portions of human PKR1 share 81% and 78% aa identity with corresponding portions of mouse PKR1 and human PKR2, with non-identity mainly in the N‑terminal sequences. Both PKR1 and PKR2 mediate the effects of prokineticins 1 and 2. This includes mitogenic regulation of angiogenesis in endocrine glands and stimulation of contraction in gastrointestinal smooth muscle.

Long Name

Prokineticin Receptor 1

Alternate Names

GPR73, GPR73a, PKR1, ProkineticinR1, PROKR1, ZAQ

Entrez Gene IDs

10887 (Human); 58182 (Mouse); 192648 (Rat)

Gene Symbol

PROKR1

UniProt

Q8TCW9

Additional Prokineticin R1/PROKR1 Products

Product Documents for Human Prokineticin R1/PKR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Prokineticin R1/PKR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Prokineticin R1/PKR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4655

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Prokineticin R1/PROKR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Prokineticin R1/PKR1 Antibody

Detection of Prokineticin R1/PKR1 in Human Monocytes by Flow Cytometry.