Human PP2C gamma Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5595N
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PP2Cγ
Met1-Asp546
Accession # O15355
Met1-Asp546
Accession # O15355
Specificity
Detects endogenous human PP2C gamma in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PP2C gamma Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: PP2C gamma
PP2C gamma (Protein phosphatase 2C gamma; also PPM1G) is a nuclear member of the metal ion-dependent PP2C family of Ser/Thr protein phosphatases. Although its predicted MW is 59 kDa, it runs anomalously at 72‑75 kDa in SDS-PAGE. PP2C gamma is widely expressed and serves as a negative regulator of the cell stress response. In particular, it dephosphorylates histones H2A and H2B, allowing for their placement into chromatin. This is important when histones are reincorporated into DNA following DNA repair. Human PP2C gamma is 546 amino acids (aa) in length. It contains a catalytic domain (aa 27‑120), an Asp/Glu-rich region (aa 258‑326) and a poly-Lys tail (aa 539‑544). One potential splice form shows a Phe substitution for aa 20‑38.
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Gene Symbol
PPM1G
UniProt
Additional PP2C gamma Products
Product Specific Notices for Human PP2C gamma Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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