Human Phospho-STAT3 (Y705) Alexa Fluor® 700-conjugated Antibody

Name: Human Phospho-STAT3 (Y705) Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
SKU: AF4607N

R&D Systems, part of Bio-Techne | Catalog # AF4607N

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human

Immunogen:

Phosphopeptide containing human STAT3 Y705 site

Applications:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label:

Alexa Fluor 700 (Excitation=675-700 nm, Emission=723 nm)

Specificity:

Detects human STAT3 when phosphorylated at Y705.

View Full Technical Details for Human Phospho-STAT3 (Y705) Alexa Fluor® 700-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT3

Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors activated in response to cytokine, growth factor, or hormone receptor signaling. Janus kinases (JAKs) phosphorylate STAT proteins and induce dimerization. Homo- or heterodimers translocate to the nucleus where they bind to DNA and activate transcription.

Item	Value
Long Name	Signal Transducer and Activator of Transcription 3
Entrez Gene IDs	6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names	APRFMGC16063, Acute-phase response factor, DNA-binding protein APRF, FLJ20882, HIES, STAT3, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)

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