Species Reactivity:

Human

Immunogen:

Phosphopeptide containing the human Lck Y394 site
Accession # P06239

Applications:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label:

Alexa Fluor 405 (Excitation=405 nm, Emission=421 nm)

Specificity:

Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.

View Full Technical Details for Human Phospho-Lck (Y394) Alexa Fluor® 405-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Lck

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene. Y394 is the major site for autophosphorylation that enhances kinase activity, while Y505 is the major phosphorylation site for negative regulation.

Item	Value
Long Name	Lymphocyte Protein Tyrosine Kinase
Entrez Gene IDs	3932 (Human); 16818 (Mouse); 313050 (Rat)
Alternate Names	EC 2.7.10, EC 2.7.10.2, Hck-3, LSK, LSTRA, Lck, Lcktkr, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, T-lymphocyte specific protein tyrosine kinase p56lck, YT16, lymphocyte-specific protein tyrosine kinase, p56(LSTRA) protein-tyrosine kinase, p56-LCK, p56lck, pp58lck, proto-oncogene tyrosine-protein kinase LCK, tyrosine-protein kinase Lck

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Product Documents

Product Datasheets

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Human Phospho-Lck (Y394) Alexa Fluor® 405-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7500V

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