Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 627945

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Product Specifications

Immunogen

Phosphopeptide containing the human LAT Y161 site
Accession # O43561

Specificity

Detects human Phospho-LAT (Y161) in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Phospho-LAT (Y161) Antibody

Detection of Human Phospho-LAT (Y161) antibody by Western Blot.

Detection of Human Phospho-LAT (Y161) by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-LAT (Y161) Monoclonal Antibody (Catalog # MAB6334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-LAT (Y161) at approximately 40 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Sheep Anti-Human LAT Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF6208). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-LAT (Y161) Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAT

LAT (Linker for Activation of T cells) is a type III transmembrane lipid raft protein that serves as a scaffold for signaling molecules. Isoforms of 262 (36 kDa) and 233, called long and short, respectively, differ by inclusion/exclusion of aa 114‑142; a 269 aa isoform lacks this region, but includes alternate N-terminal sequence. Upon T-cell antigen receptor activation, LAT is multiply phosphorylated by ZAP-70/Syk protein tyrosine kinases, creating docking sites for SH2 domain-containing proteins. Phospholipase C-gamma docks at Y161, which is pY132 in the short form of LAT. Mutation of this site results in a Th2 autoimmune lymphoproliferative disorder in mice.

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Entrez Gene IDs

27040 (Human); 16797 (Mouse); 81511 (Rat)

Gene Symbol

LAT

UniProt

O43561 (Human)

Additional LAT Products

Product Documents for Human Phospho-LAT (Y161) Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-LAT (Y161) Antibody

For research use only

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