Human Pax3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2457
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Avian - Chicken
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pax3
Met1-Ser215
Accession # NP_000429
Met1-Ser215
Accession # NP_000429
Specificity
Detects Pax3 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5 and 1% rhPax7, under reducing conditions, is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Pax3 Antibody
Detection of Pax3 in Human Skin.
Pax3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Pax3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2457) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus of melanocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Pax3 Antibody
Application
Recommended Usage
Immunohistochemistry
3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
0.1 µg/mL
Sample: Recombinant Human Pax3
Sample: Recombinant Human Pax3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax3
Long Name
Paired Box Gene 3 [Waardenburg Syndrome 1]
Alternate Names
HUP2, WS1
Gene Symbol
PAX3
UniProt
Additional Pax3 Products
Product Documents for Human Pax3 Antibody
Product Specific Notices for Human Pax3 Antibody
For research use only
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