Pancreatic Polypeptide/PP was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Pancreatic Polypeptide/ PP Monoclonal Antibody (Catalog # MAB62971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Pancreatic Polypeptide/PP Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas

Reviewed Applications

Read 1 review rated 5 using MAB62971 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pancreatic Polypeptide/PP

Pancreatic Polypeptide (PP) is an 11 kDa (calculated), unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the 36 aa PP hormone, a 20 aa icosapeptide of unknown function, and a C-terminal prosequence (1). PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake (2). The human PP prohormone shares 57% and 55% aa identity with mouse and rat PP, respectively.

References

Leiter A, et al. (1984) J. Biol. Chem. 259:14702.
Asakawa, A et al. (2003) Gastroenterology 124:1325.

Alternate Names

INN, Obinepitide, PNP, PP, PPY

Entrez Gene IDs

5539 (Human)

Gene Symbol

PPY

UniProt

P01298

Additional Pancreatic Polypeptide/PP Products

Product Documents for Human Pancreatic Polypeptide/PP Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Pancreatic Polypeptide/PP Antibody

For research use only

Citations
Reviews

Human Pancreatic Polypeptide/PP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB62971

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Pancreatic Polypeptide/PP Antibody

Pancreatic Polypeptide/PP in Human Pancreas.