Human p53 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC13551A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 184727
Product Specifications
Immunogen
E. coli-derived recombinant human p53
Asp7-Asp393
Accession # P04637
Asp7-Asp393
Accession # P04637
Specificity
Detects human p53 in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human p53 APC-conjugated Antibody
Detection of p53 in MCF-7 cells by Flow Cytometry
MCF-7 cells were stained with Mouse Anti-Human p53 APC-conjugated Monoclonal Antibody (Catalog # IC13551A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.Applications for Human p53 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Sample: MCF‑7 human breast cancer cell line fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer kit
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: p53
The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human p53 APC-conjugated Antibody
Product Specific Notices for Human p53 APC-conjugated Antibody
For research use only
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