Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/ml of Rabbit Anti-Human NSF Monoclonal Antibody (Catalog # MAB11766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NSF at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human NSF Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NSF

N-ethylmaleimide-sensitive factor (NSF) is an ATPase of the AAA+ protein family, with a molecular weight of approximately 83 kDa. NSF is a key component of membrane trafficking pathways and plays a central role in the disassembly of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, thereby facilitating vesicle fusion and neurotransmitter release. It is ubiquitously expressed across cell types and is essential for intracellular trafficking processes such as endocytosis, exocytosis, and autophagosome formation. NSF's functionality extends to maintaining the proper organization and dynamics of intracellular membranes, making it crucial for cellular homeostasis. Dysregulation of NSF activity has been implicated in various pathological conditions, including neurodegenerative disorders, where defects in vesicle fusion processes are linked to cognitive impairment. Additionally, NSF influences the recycling of neurotransmitter receptors and may play a role in synaptic plasticity, highlighting its importance in neuronal function and its potential as a therapeutic target for neurological diseases.

References

Hanson PI, Whiteheart SW. AAA+ proteins: have engine, will work. Nat Rev Mol Cell Biol. 2005 Jul;6(7):519-29. doi: 10.1038/nrm1684. PMID: 16072036.
Yang J, Kong L, Zou L, Liu Y. The role of synaptic protein NSF in the development and progression of neurological diseases. Front Neurosci. 2024 Oct 21;18:1395294. doi: 10.3389/fnins.2024.1395294. PMID: 39498393; PMCID: PMC11532144.
Zhao M, Brunger AT. Recent Advances in Deciphering the Structure and Molecular Mechanism of the AAA+ ATPase N-Ethylmaleimide-Sensitive Factor (NSF). J Mol Biol. 2016 May 8;428(9 Pt B):1912-26. doi: 10.1016/j.jmb.2015.10.026. Epub 2015 Nov 3. PMID: 26546278; PMCID: PMC4854814.

Long Name

N-ethylmaleimide sensitive factor, vesicle fusing ATPase

Alternate Names

DEE96, SEC18, SKD2

Entrez Gene IDs

4905 (Human); 18195 (Mouse); 60355 (Rat)

Gene Symbol

NSF

UniProt

P46459 (Human)

Additional NSF Products

Product Documents for Human NSF Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NSF Antibody

For research use only

Citations
Reviews

Human NSF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11766

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NSF Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human NSF Antibody

Detection of Human NSF by Western Blot.