Human Notch-2 Intracellular Domain Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3735AFP647

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FAB3735AFP647-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 411801

View all Notch-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Notch-2

Specificity

Detects human Notch-2 intracellular domain (ICD) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Notch-1 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch‑4 ICD.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Notch-2

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins.  It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180 kDa disulfide-linked extracellular domain (ECD) (likely aa 25-1538) and a 110 kDa membrane bound truncated segment (likely aa 1539-2471).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa 1697-2471), a potential nuclear transcription factor.  In the nucleus, NICD regulates CSL pathways.  When NICD is phosphorylated, it is deactivated.  Over aa 2063-2413, human Notch-2 is 94% and 89% aa identical to dog and mouse Notch-2, respectively.   

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Gene Symbol

NOTCH2

UniProt

Q04721.3

Additional Notch-2 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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