Detects human NETO1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human NETO2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human NETO1 Antibody

NETO1 in MOLT‑4 Human Cell Line.

NETO1 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

NETO1 in Human Prostate.

NETO1 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human NETO1 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

0.1 µg/mL
Sample: Recombinant Human NETO1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NETO1

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511 amino acid residue mature human NETO1 contains an extracellular domain with two CUB domains (aa 41‑155 and 172‑287), and one class A LDL-receptor segment (aa 292‑326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. Over aa 23‑345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively.

Long Name

Neuropilin [NRP] And Tolloid [TLL]-like 1

Alternate Names

BCTL1, BTCL1

Entrez Gene IDs

81832 (Human); 246317 (Mouse); 307206 (Rat)

Gene Symbol

NETO1

UniProt

Q8TDF5

Additional NETO1 Products

Product Documents for Human NETO1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human NETO1 Antibody

For research use only

Citations
Reviews

Human NETO1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4337

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NETO1 Antibodies »

Product Specifications

Immunogen

Specificity