Human MyD88 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2928
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 316603
Product Specifications
Immunogen
E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836
Met1-Pro296
Accession # Q99836
Specificity
Detects human MyD88 in direct ELISAs. Does not cross-react with mouse MyD88.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human MyD88 Antibody
MyD88 in Raji Human Cell Line.
MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human MyD88 Monoclonal Antibody (Catalog # MAB2928) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of MyD88 by Western Blot
Expression of MyD88 in normal and degenerated disc. (A) The correlations of the expression of the MyD88 with IVDD grades by linear regression analysis. (“0.00” means early stage-thompson grade III, IV, “1.00” means advanced stage-thompson grade V). (B) Detection of MyD88 levels in normal and degenerated disc by western blot. ***P < 0.001. (C) Double-staining of RIP3 with MyD88 in NP cells in normal and degenerated disc. (D) Relative IF intensity of MyD88 in NP cells. ***P < 0.001. N = 6. Bar = 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36213280), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MyD88 by Western Blot
Expression of MyD88 in normal and degenerated disc. (A) The correlations of the expression of the MyD88 with IVDD grades by linear regression analysis. (“0.00” means early stage-thompson grade III, IV, “1.00” means advanced stage-thompson grade V). (B) Detection of MyD88 levels in normal and degenerated disc by western blot. ***P < 0.001. (C) Double-staining of RIP3 with MyD88 in NP cells in normal and degenerated disc. (D) Relative IF intensity of MyD88 in NP cells. ***P < 0.001. N = 6. Bar = 50μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36213280), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human MyD88 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MyD88
MyD88 was first identified as a myeloid differentiation primary response protein. It contains a cytoplasmic Toll/interleukin 1 receptor (TIR) domain that classifies it as a member of the TIR superfamily. MyD88 is expressed in many adult tissues including heart, kidney and liver tissue and in monocyte, T, B, NK and dendritic cells.
Long Name
Myeloid Differentiation Primary Response Gene 88
Alternate Names
MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88
Gene Symbol
MYD88
UniProt
Additional MyD88 Products
Product Documents for Human MyD88 Antibody
Product Specific Notices for Human MyD88 Antibody
For research use only
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