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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-reported, Immunohistochemistry, Knockout Validated, Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 867711

Product Specifications

Immunogen

E. coli-derived recombinant human YAP1
Ala347-Leu504
Accession # P46937

Specificity

Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human/Mouse/Rat YAP1 Alexa Fluor® 532-conjugated Antibody

Application
Recommended Usage

CyTOF-reported

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: YAP1

YAP1 (Yes-associated protein 1), also called YAP65 or Yorkie Homolog, is a widely expressed 65 kDa cytoplasmic and nuclear transcriptional regulator protein in the Hippo pathway that regulates organ size. The 504 amino acid (aa) human YAP1 contains two WW (tryptophan-containing protein binding) domains (aa 171-263), a transactivation domain (aa 219-504) and 19 sites that may be phosphorylated by kinases such as LATS1 and 2, MAPK8 and 9, CK1, or ABL1. Isoforms of 488, 454 and 326 aa lack aa 328-343, 230-267 (plus a 3 aa substitution for aa 329-343), or 1-178, respectively. YAP1 binds the Yes tyrosine kinase SH3 domain and many other proteins, notably ErbB4. YAP1 expression is increased in some liver and prostate cancers. Within aa 347-504, human YAP1 shares 94% and 93% aa sequence identity with mouse and rat YAP1, respectively.

Long Name

Yes-associated Protein 1

Alternate Names

YAP2, YAP65, YKI, Yorkie Homolog

Entrez Gene IDs

10413 (Human); 22601 (Mouse)

Gene Symbol

YAP1

UniProt

Additional YAP1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat YAP1 Alexa Fluor® 532-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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