Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Ras-GAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ras-GAP at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Ras-GAP Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ras-GAP

Ras-GAP (Ras GTPase-activating protein) interacts with Ras to stimulate the otherwise weak intrinsic GTPase activity of Ras and to promote the return of Ras to an inactive GDP-bound state. Thus, Ras-GAP is a negative regulator of Ras. Human Ras-GAP is 1047 amino acids in length. The N-terminal region contains two SH2 domains and an intervening SH3 domain, while the C-terminal half of Ras-GAP is sufficient to accelerate Ras GTPase activity.

Long Name

GTPase activating protein

Alternate Names

CM-AVM, PKWS, RASA1, RasGAP

Entrez Gene IDs

5921 (Human); 218397 (Mouse); 25676 (Rat)

Gene Symbol

RASA1

UniProt

P20936

Additional Ras-GAP Products

Product Documents for Human/Mouse/Rat Ras-GAP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat Ras-GAP Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat Ras-GAP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5094

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Ras-GAP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat Ras-GAP Antibody

Detection of Human/Mouse/Rat Ras-GAP by Western Blot.