Detects human, mouse and rat RalA in Western blots. When immunoblotted, no cross-reactivity with recombinant human (rh) RalB, rhH-Ras, rhN‑Ras or rhK-Ras is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat RalA Antibody

Detection of Human/Mouse/Rat RalA by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human RalA and RalB (1 ng/lane) were included. A specific band for RalA was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human RalA by Simple Western^TM.

Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and MDA-MB-468 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for RalA at approximately 32 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat RalA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3270). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Human/Mouse/Rat RalA Antibody

Application

Recommended Usage

Simple Western

20 µg/mL
Sample: Exosome Standards (Human Urine) (Catalog # NBP2-49840) and MDA-MB-468 human breast cancer cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RalA

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Long Name

Ras-like Protein A

Alternate Names

Rasl1

Entrez Gene IDs

5898 (Human); 56044 (Mouse); 81757 (Rat)

Gene Symbol

RALA

UniProt

P11233

Additional RalA Products

Product Documents for Human/Mouse/Rat RalA Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat RalA Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat RalA Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3270

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity