Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PKA Ca/ beta was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human PKA C alpha/ beta by Western Blot.

Western blot shows Recombinant Human Active PKA Ca (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (1 ng/lane each). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse/Rat PKA C alpha/ beta Antibody

Application

Recommended Usage

Western Blot

0.2 µg/mL
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA C alpha/beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit alpha/beta

Alternate Names

PRKACA/B

Entrez Gene IDs

5566 (Human); 18747 (Mouse); 25636 (Rat)

Gene Symbol

PRKACA

UniProt

P17612 (Human)

Additional PKA C alpha/beta Products

Product Documents for Human/Mouse/Rat PKA C alpha/ beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat PKA C alpha/ beta Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat PKA C alpha/ beta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5908

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat PKA C alpha/ beta Antibody

Detection of Human/Mouse/Rat PKA C alpha/ beta by Western Blot.