Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 647-conjugated Antibody

Name: Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: AF1018R

R&D Systems, part of Bio-Techne | Catalog # AF1018R

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Phosphopeptide containing ERK1 T202/Y204 sites

Applications:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label:

Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)

Specificity:

Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.

View Full Technical Details for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Alexa Fluor® 647-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

Item	Value
Long Name	Extracellular Signal-regulated Kinase 1
Entrez Gene IDs	5595 (Human); 26417 (Mouse); 50689 (Rat)
Alternate Names	EC 2.7.11, ERK-1, ERK1, ERK1p44-MAPK, ERT2, Extracellular signal-regulated kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, MGC20180, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, P44ERK1, PRKM3, PRKM3EC 2.7.11.24, extracellular signal-related kinase 1, mitogen-activated protein kinase 3, p44-ERK1, p44mapk

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