Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF887AFP555

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Rabbit IgG

View all Akt Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human, mouse, and rat Akt1 S473 site

Specificity

Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Akt

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog

Alternate Names

PKB, RAC

Additional Akt Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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