Human/Mouse/Rat p62/SQSTM1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8028R
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Knockout Validated, Western Blot, Immunocytochemistry, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # 864807R
Product Specifications
Immunogen
E. coli-derived recombinant human p62/SQSTM1
Asp368-Leu440
Accession # Q13501
Asp368-Leu440
Accession # Q13501
Specificity
Detects human p62/SQSTM1 in ELISAs. Detects human, mouse and rat p62/SQSTM1 in Western blots
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat p62/SQSTM1 Antibody
Detection of Human and Mouse p62/SQSTM1 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Monoclonal Antibody (Catalog # MAB8028R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for p62/SQSTM1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p62/SQSTM1 in HeLa Human Cell Line.
p62/SQSTM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with staurosporine using Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Monoclonal Antibody (Catalog # MAB8028R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human p62/SQSTM1 Specificity Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat p62/SQSTM1 Monoclonal Antibody (Catalog # MAB8028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for p62/SQSTM1 at approximately 62 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat p62/SQSTM1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with staurosporine
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with staurosporine
Immunoprecipitation
1 µg/1 mg cell lysate
Sample: Cell lysate of U2OS human osteosarcoma cell line
Sample: Cell lysate of U2OS human osteosarcoma cell line
Knockout Validated
p62/SQSTM1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line and parental U2OS cell line but is not detectable in p62/SQSTM1 knockout HeLa cell line and knockout U2OS cell line.
Western Blot
1 µg/mL
Sample: A172 human glioblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Sample: A172 human glioblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p62/SQSTM1
Long Name
Sequestosome 1
Alternate Names
A170, EBIAP, ORCA, OSIL, PDB3, Sequestosome 1, SQSTM1, ZIP3
Gene Symbol
SQSTM1
UniProt
Additional p62/SQSTM1 Products
Product Documents for Human/Mouse/Rat p62/SQSTM1 Antibody
Product Specific Notices for Human/Mouse/Rat p62/SQSTM1 Antibody
For research use only
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