Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MIOS Monoclonal Antibody (Catalog # MAB9289) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MIOS at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat MIOS Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIOS

MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex. MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3. This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids. Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain. Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.

Long Name

Missing Oocyte, Meiosis Regulator

Alternate Names

MIO

Entrez Gene IDs

54468 (Human); 252875 (Mouse); 362324 (Rat)

Gene Symbol

MIOS

UniProt

Q9NXC5

Additional MIOS Products

Product Documents for Human/Mouse/Rat MIOS Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat MIOS Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat MIOS Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9289

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all MIOS Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat MIOS Antibody

Detection of Human MIOS by Western Blot.