Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4498) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK1 at approximately 63 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat GRK1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, PT18 mouse mast/basophil cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK1

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK1 and GRK7 are members of the rhodopsin kinase or visual GRK subfamily.

Long Name

G protein-coupled Receptor Kinase 1

Alternate Names

GPRK1, RHOK

Entrez Gene IDs

6011 (Human); 24013 (Mouse); 81760 (Rat)

Gene Symbol

GRK1

UniProt

Q15835

Additional GRK1 Products

Product Documents for Human/Mouse/Rat GRK1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse/Rat GRK1 Antibody

For research use only

Citations
Reviews

Human/Mouse/Rat GRK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4498

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GRK1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse/Rat GRK1 Antibody

Detection of Human/Mouse/Rat GRK1 by Western Blot.