Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

Detection of A2B5 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Biotinylated Monoclonal Antibody (Catalog # BAM1416), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line treated with retinoic acid

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References

Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Alternate Names

A2B5 marker

Additional A2B5 Products

Product Documents for Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1416

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications