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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 715901

Product Specifications

Immunogen

E. coli-derived recombinant human ARK5/NUAK1
Lys311-Met462
Accession # O60285

Specificity

Detects human ARK5/NUAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SNARK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ARK5/NUAK1

AMPK-related kinase 5 (ARK5), also known as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short isoform of the human protein is prematurely tuncated within this domain. Within aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5, respectively.

Long Name

AMPK-related Protein Kinase 5

Alternate Names

NUAK1

Entrez Gene IDs

9891 (Human); 77976 (Mouse); 299694 (Rat)

Gene Symbol

NUAK1

UniProt

Additional ARK5/NUAK1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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