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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Rab7a
Thr2-Ser204
Accession # P51149

Specificity

Detects human and mouse Rab7a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Rab9a and rhRab9b is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse Rab7a Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Rab7a

Rab7a (Ras-related protein Rab 7A; also RAB7) is a 22-24 kDa member of the Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as skeletal muscle, neurons, osteoclasts and macrophages. Rab7a plays a key role in the formation of phagolysosomes. In particular, Rab7a-GTP becomes associated with early phagosomes. The GTP form of Rab7a first recruits RILP, a molecule that interacts with ORPL1 and promotes the formation of a dynactin:dynein complex. This complex now initiates the movement of the phagosome along microtubules, where it fuses with lysosomes and endosomes at select points, creating phagolysosomes. Human Rab7a is 207 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C‑terminal CXC prenylation sequence (aa 205-207). There is one utilized phosphorylation site at Tyr183. Full-length human and mouse Rab7a have identical aa sequences. Notably, however, point mutations with single aa changes are noted in both species. By contrast, human Rab7a and Rab7b share less than 50% aa sequence identity, each representing the product of distinct genes.

Long Name

RAs Genes from Brain Protein 7a

Alternate Names

PRO2706, RAB7

Entrez Gene IDs

7879 (Human); 19349 (Mouse); 29448 (Rat)

Gene Symbol

RAB7A

UniProt

Additional Rab7a Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Rab7a Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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