Human/Mouse Pygopus-1 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3617U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 466023
Product Specifications
Immunogen
E. coli-derived recombinant human Pygopus‑1
Ala2-Ala419
Accession # Q9Y3Y4
Ala2-Ala419
Accession # Q9Y3Y4
Specificity
Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human/Mouse Pygopus-1 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Pygopus-1
Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta-catenin in the nucleus during Wnt-signaling. In particular, following beta-catenin activation and translocation to the nucleus, beta-catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta-catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.
Alternate Names
Pygo1, Pygopus1
Gene Symbol
PYGO1
UniProt
Additional Pygopus-1 Products
Product Specific Notices for Human/Mouse Pygopus-1 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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