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Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Catalog # IC1759A | R&D Systems, Inc. a Bio-Techne Brand
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IC1759A-025
IC1759A-100

Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 240408

Product Summary for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Immunogen

E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860

Specificity

Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Detection of Oct‑3/4 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
Detection of Oct‑3/4 antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Oct‑3/4 in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line treated with 5 µM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct‑3/4 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Rat Anti-Human/Mouse Oct‑3/4 APC‑conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using IC1759A in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References

  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Gene Symbol

POU5F1

UniProt

Product Documents for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Oct‑3/4 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse Oct‑3/4 APC‑conjugated Antibody (4)

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