Human/Mouse IL-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB405
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Guinea Pig
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Immunoassay, Neutralization, ELISA Development, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # TRFK5
Product Specifications
Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Specificity
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human/Mouse IL-5 Antibody
Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody.
Recombinant Mouse IL-5 (405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically 0.004-0.015 µg/mL.
Human IL-5 ELISA Standard Curve
Recombinant Human IL-5 (Catalog # 205-IL) was serially diluted and captured by Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human IL-5 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse IL-5 ELISA Standard Curve
Recombinant Mouse IL-5 (Catalog # 405-ML) was serially diluted and captured by Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-5 Monoclonal Antibody (Catalog # MAB705) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human/Mouse IL-5 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.
Human IL-5 Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Mouse IL-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB405 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
Additional IL-5 Products
Product Documents for Human/Mouse IL-5 Antibody
Product Specific Notices for Human/Mouse IL-5 Antibody
For research use only
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