Human/Mouse DACT3/Dapper 3 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5767AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: DACT3/Dapper 3
Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins. It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta-catenin signaling. When present in colorectal tumor cells, it initiates apoptosis. Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta-catenin entry into the nucleus. Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610). There is one potential splice variant that shows a 45 aa substitution for aa 413-610. In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.
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Long Name
Dapper, Antagonist of beta-Catenin, Homolog 3
Alternate Names
Dapper 3, RRR1
Gene Symbol
DACT3
UniProt
Additional DACT3/Dapper 3 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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