Human/Mouse DACH2 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5230S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DACH2
Gln419-Gln583
Accession # Q96NX9
Gln419-Gln583
Accession # Q96NX9
Specificity
Detects human and mouse DACH2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human/Mouse DACH2 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: DACH2
DACH2 (Dachshund 2) is a 65 kDa (predicted) member of the DACH family of transcription factors. It is expressed in the dermomyotome and Mullerian duct, and promotes Mullerian duct formation and myogenin gene suppression. Human DACH2 is 599 amino acids (aa) in length and contains a DD1 domain in the N-terminus (aa 66‑162) that mediates DNA binding, and a C-terminal, coiled-coil DD2 domain (aa 452‑543) that interacts with EYA proteins. Multiple splice variants of DACH2 are known. There is an alternate start site at Met220 that may be accompanied by both a deletion of aa 259‑311 and a three aa substitution for aa 585‑599. In addition, there is a deletion of aa 163‑175 that may be accompanied by an Ala substitution for aa 584‑599. Finally, there is a 41 aa substitution for aa 1‑175. Over aa 419‑583, human DACH2 is 93% aa identical to mouse DACH2.
Long Name
Dachshund Homolog 2
Alternate Names
Dach2, dachshund homolog 2, dachshund homolog 2 (Drosophila), FLJ31391, MGC138545
Gene Symbol
DACH2
UniProt
Additional DACH2 Products
Product Specific Notices for Human/Mouse DACH2 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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