Human/Mouse COX-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37401
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 596002
Product Specifications
Immunogen
E.coli-derived recombinant human COX-1
Asn381-Leu599
Accession # P23219
Asn381-Leu599
Accession # P23219
Specificity
Detects human COX-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) COX-4I1 and no cross-reactivity with rhCOX-1 or rhCOX-4I2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse COX-1 Antibody
Detection of Human and Mouse COX‑1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and B16-F10 mouse melanoma cell line. PVDF Membrane was probed with 2 µg/mL of Human COX-1 Monoclonal Antibody (Catalog # MAB37401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse COX-1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and B16‑F10 mouse melanoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and B16‑F10 mouse melanoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COX-1
COX-1 is an approximately 70 kDa protein that is associated with microsomal membranes. COX-1 participates in the synthesis of prostaglandins and is the enzymatic target of non-steroidal anti-inflammatory drugs. A splice variant of COX-1 is generated by internal deletion of 37 residues. Human COX-1 shares 88%‑90% amino acid sequence identity with mouse and rat COX‑1.
Long Name
Cyclooxygenase 1
Alternate Names
COX1, PTGS1
Gene Symbol
PTGS1
UniProt
Additional COX-1 Products
Product Documents for Human/Mouse COX-1 Antibody
Product Specific Notices for Human/Mouse COX-1 Antibody
For research use only
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