Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Bcl-6 by Simple Western^TM.

Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-6 at approximately 111 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Bcl-6 Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-6

Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.

Long Name

B Cell Lymphoma/Leukemia 6

Alternate Names

Bcl-5, Bcl6, LAZ3, ZBTB27, ZNF51

Entrez Gene IDs

604 (Human); 12053 (Mouse); 303836 (Rat)

Gene Symbol

BCL6

UniProt

Q8CB25

Additional Bcl-6 Products

Product Documents for Human/Mouse Bcl-6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Bcl-6 Antibody

For research use only

Citations
Reviews

Human/Mouse Bcl-6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5046

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Bcl-6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Bcl-6 Antibody

Detection of Mouse and Human Bcl‑6 by Western Blot.

Detection of Human Bcl-6 by Simple WesternTM.