Western blot shows lysates of A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bad at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Bad in Jurkat Human Cell Line.

Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Rabbit Anti-Human/Mouse Bad Antigen Affinity-purified Polyclonal Antibody (Catalog # AF819) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human/Mouse Bad aa 1‑21 Antibody

Application

Recommended Usage

Immunocytochemistry

0.3-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and L‑929 mouse fibroblast cell line

Reviewed Applications

Read 2 reviews rated 4 using AF819 in the following applications:

Microarray (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bad

Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.

Long Name

Bcl-xL/Bcl-2 Associated Death Promoter

Alternate Names

BBC6, BCL2L8

Entrez Gene IDs

572 (Human); 12015 (Mouse); 64639 (Rat)

Gene Symbol

BAD

UniProt

Q92934 (Human)

Additional Bad Products

Product Documents for Human/Mouse Bad aa 1‑21 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Bad aa 1‑21 Antibody

For research use only

Citations
Reviews

Human/Mouse Bad aa 1‑21 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF819

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Bad aa 1‑21 Antibody

Detection of Human/Mouse Bad by Western Blot.