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Human/Mouse ATG12 Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6807AFP555

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FAB6807AFP555-100UG

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 628902

Product Specifications

Immunogen

E. coli-derived recombinant human ATG12

Specificity

Detects human ATG12 in direct ELISAs, and human and mouse ATG12 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ATG12

ATG12 is a 15 kDa cytosolic protein with a C-terminal ubiquitin-like domain. The conjugation of ATG12 to ATG5 is required for the autophagosome pathway of bulk degradation. The ATG5:ATG12 heterodimer associates noncovalently with an ATG16 multimer to generate autophagosomes. The ATG5:ATG12 conjugate also inhibits type I interferon-induced antiviral responses and promotes the lipidation of ATG8. Alternate splicing of human ATG12 generates an isoform that lacks the ubiquitin-like domain. Human ATG12 shares 89% and 85% aa sequence identitiy with mouse and rat ATG12, respectively.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Entrez Gene IDs

9140 (Human); 67526 (Mouse); 361321 (Rat)

Gene Symbol

ATG12

UniProt

Additional ATG12 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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