Western blot shows lysates of A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse AMPK beta2 Monoclonal Antibody (Catalog # MAB3808) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human AMPK beta2 (0.5 ng) was included. A specific band for AMPK beta 2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse AMPK beta2 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK beta 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha1, alpha2, beta1, beta2, gamma1, gamma2, gamma3), with all 12 combinations able to form complexes. The beta1 subunit is expressed at higher levels than the beta2subunit in liver, while beta2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 2

Alternate Names

PRKAB2

Entrez Gene IDs

5565 (Human); 108097 (Mouse); 64562 (Rat)

Gene Symbol

PRKAB2

UniProt

O43741

Additional AMPK beta 2 Products

Product Documents for Human/Mouse AMPK beta2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse AMPK beta2 Antibody

For research use only

Citations
Reviews

Human/Mouse AMPK beta2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3808

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all AMPK beta 2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse AMPK beta2 Antibody

Detection of Human/Mouse AMPK beta2 by Western Blot.