Detects human, mouse, and rat 14-3-3 zeta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human 14‑3‑3 beta, epsilon, eta, gamma, or sigma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse 14-3-3 zeta Antibody

Detection of Human/Mouse 14‑3‑3 zeta by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheo-chromocytoma cell line. PVDF membrane was probed with 0.25 µg/mL Goat Anti-Human/ Mouse 14-3-3 zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2669) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, and zeta (2 ng/lane) were included. A specific band for 14-3-3 zeta was detected at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse 14-3-3 zeta Antibody

Application

Recommended Usage

Western Blot

0.25 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 1 review rated 4 using AF2669 in the following applications:

Simple Western (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 zeta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha/ beta, epsilon, eta, gamma, tau/ theta, sigma, and zeta/ delta). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (YWHAZ) is a 245 amino acid, 27 kDa protein.

Alternate Names

1433 zeta, KCIP-1, YWHAZ

Entrez Gene IDs

7534 (Human)

Gene Symbol

YWHAZ

UniProt

P63104

Additional 14-3-3 zeta Products

Product Documents for Human/Mouse 14-3-3 zeta Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human/Mouse 14-3-3 zeta Antibody

For research use only

Citations
Reviews

Human/Mouse 14-3-3 zeta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2669

Key Product Details

Species Reactivity

Validated:

Cited:

Applications