Human MMP-9 Antibody

MMP‑9 in MMP‑9 transfected NS0 Mouse Cell Line.

MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MMP‑9 in Human Liver Cancer Tissue.

MMP-9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of MMP-9 by Western Blot

Neutrophil degranulation is increased in response to C3d treatment. Neutrophils isolated from HC individuals were incubated at 37 °C and remained unstimulated (Con) or were stimulated with C3d (2–40 µg/mL) or TNF-alpha (10 ng / 2 × 107 cells) (A–C). (A) Cell-free supernatants were collected at 10 min and immunoblotted for markers of tertiary granule (MMP-9), secondary granule (hCAP-18), or primary granule (BPI) release. Densitometry of MMP-9 (B), hCAP-18 (C), and BLPI (D) immunobands was performed. Western blot analyses of whole cell lysates demonstrated equal expression levels of beta-actin, confirming the use of equal cell numbers per reaction. C3d-challenged neutrophils released significantly greater levels of all three granule types when compared to untreated controls (Con) (n = 5 biological repeats, ANOVA followed by Bonferroni post-hoc test for selected groups). All results are expressed as relative densitometry units (DU), with representative Western blots presented. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34944741), licensed under a CC-BY license. Not internally tested by R&D Systems.