Human Meprin alpha Subunit/MEP1A Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3220AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-ready
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 364312
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
Specificity
Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEP1B or recombinant mouse MEP1A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Meprin alpha Subunit/MEP1A
Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid (aa) sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). Human Meprin alpha subunit consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 65), and a mature chain (aa 66 to 746) containing the following domains: catalytic (aa 62 to 263), MAM (aa 264 to 433), MATH (aa 434 to 593), EGF-like (aa 670 to 710), transmembrane (aa 713 to 740), and cytoplasmic (aa 741 to 746).
References
- Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
- Stocker, W. et al. (1995) Protein Sci. 4:823.
- Bertenshaw, G.P., et al. (2001) J. Biol. Chem. 276:13248.
- Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
Alternate Names
MEP1A, PPHA
Gene Symbol
MEP1A
UniProt
Additional Meprin alpha Subunit/MEP1A Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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