Detects human MAdCAM-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse MAdCAM-1, recombinant human (rh) ALCAM, rhBCAM, rhCEACAM-1, rhEpCAM, rhICAM-1, -2, -3, -4, -5, rhCD31/PECAM-1, or rhVCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human MAdCAM-1 APC-conjugated Antibody

Detection of MAdCAM-1 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MAdCAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB6056A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human MAdCAM-1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HeLa human cervical epithelial carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MAdCAM-1

Mucosal Addressin Cell Adhesion Molecule-1 (MAdCAM-1) is an approximately 60 kDa type 1 transmembrane glycoprotein. It is an endothelial cell adhesion molecule that belongs to the immunoglobulin (Ig) superfamily of proteins (1). Human MAdCAM-1 is synthesized as a 382 amino acid (aa) precursor that contains an 18 aa signal sequence, a 299 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD there is one potential site for N-linked glycosylation (2). The ECD comprises two Ig-like domains of 90 aa and 119 aa, respectively, each possessing invariant cysteine residues that stabilize the Ig loop (2). There is also a Ser-Thr-Pro-rich (71%) mucin-like 48 aa domain that is (aa 206‑317) formed by six tandem repeats of an eight aa sequence having the general consensus DTTSPEP/SP. This mucin domain contains 19 potential sites for O-linked glycosylation (2, 3). A splicing variant in which a single Ala residue is substituted for aa 223‑334 in isoform 1 produces a second isoform. Human mature MAdCAM-1 shares only 44% aa sequence identity with mature mouse MAdCAM-1. The integrin alpha(4) beta(7), which is expressed on lymphocytes, functions as the MAdCAM-1 receptor (1). The Ig domains of MAdCAM-1 are critical to alpha(4) beta(7) binding, and the mucin domain has activity in L‑Selectin binding. MAdCAM-1 expression is up-regulated by TNF-alpha and IL‑1 beta. MAdCAM-1 is expressed on the surface of High Endothelial Venules (HEV) in the gut and in Peyer’s patches, on endothelial cells of the mesenteric lymph nodes, lamina propria of the small and large intestine, and the mammary gland during lactation, and on brain endothelial cells (1). MAdCAM‑1 has also been reported to be expressed in the liver portal region in autoimmune hepatitis (1), and in bone marrow following allogenic (genetically non-identical) hematopoietic stem cell transplantation, where it recruits donor T cells, which may lead to graft versus host disease (3, 4). MAdCAM‑1 functions as a homing receptor, and plays a central role in leukocyte migration into HEVs and Peyer’s patch (5). In addition to its normal role in lymphocyte trafficking to mucosal tissue, MAdCAM‑1 expression is also dramatically increased in chronic inflammatory and disease states (1, 6), including inflammatory bowel disease (Crohn’s disease and ulcerative colitis) (7), sclerosing cholangitis (8), and diabetes (9), and may play an important role in these conditions.

References

Ando, T. et al. (2007) BMC Physiol. 7:10.
Dando, J. et al. (2002) Acta Crystallogr. D 58:233.
Leung, E. et al. (1996) Immunol. Cell Biol. 74:490.
Ambruzova, Z. et al. (2009) Hum. Immunol. 70:457.
Tada, T. et al. (2008) Exp. Anim. 57:247.
Volpes, R. et al. (1992) Hepatology 15:269.
Connor, E.M. et al. (1999) J. Leukoc. Biol. 65:349.
Ala, A. et al. (2001) Gut 49:3043.
Yang, X.D. et al. (1997) Diabetes 46:1542.

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Entrez Gene IDs

8174 (Human); 17123 (Mouse)

Gene Symbol

MADCAM1

UniProt

AAY82472

Additional MAdCAM-1 Products

Product Documents for Human MAdCAM-1 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MAdCAM-1 APC-conjugated Antibody

For research use only

Citations
Reviews

Human MAdCAM-1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6056A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all MAdCAM-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human MAdCAM-1 APC-conjugated Antibody

Detection of MAdCAM-1 in HeLa Human Cell Line by Flow Cytometry.

Applications for Human MAdCAM-1 APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: MAdCAM-1

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional MAdCAM-1 Products

Product Documents for Human MAdCAM-1 APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human MAdCAM-1 APC-conjugated Antibody