Western blot shows lysates of JAR human choriocarcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN-28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LIN‑28A in BG01V Human Stem Cells.

LIN-28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LIN‑28A in D3 Mouse Stem Cells.

LIN-28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human LIN-28A by Simple Western^TM.

Simple Western lane view shows lysates of NTera-2 human testicular embryonic carcinoma cell line and JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LIN-28A at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human LIN-28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of LIN-28A by Western Blot

Expression of LIN28 in mouse testis. Western blot analysis was performed on 20 μg of protein extracts for each sample. beta-actin served as a control. Molecular weight standards were marked in kDa. (A) Western blot analysis of LIN28 in adult mouse tissues. (B) Absence of LIN28 in germ cell-deficient XXY* testes. Testes were collected from adult and post-natal day 10-old mice. V6.5 mouse embryonic stem (ES) cells served as a positive control. LIN28 was absent in fibroblast feeder cells. (C) Developmental expression of LIN28 in postnatal testes. Testes were collected from mice of postnatal day 1 through adulthood. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19563657), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of LIN-28A by Western Blot

Expression and siRNA knockdown of LIN28 in cultured spermatogonia highly enriched for spermatogonial stem cells (SSCs). (A) Immunostaining of SSCs with anti-LIN28 and anti-PLZF or anti-GFRA1 antibodies. Scale bar, 50 μm. (B) Quantitative PCR measurement of Lin28 mRNA levels (n = 3, mean ± SE) in SSCs after siRNA treatment for 30 hours. (C) Decreased LIN28 protein abundance (43% compared to the control) in SSCs after 30 hours of siRNA treatment. The control SSCs were not treated with Lin28 siRNA. Feeder cells served as a negative control. beta-actin served as a loading control. (D) The number of SSCs (n = 3, mean ± SE) with and without Lin28 siRNA treatment. (E) Quantitative measurement of mature let-7g miRNA levels (n = 3, mean ± SE) in SSCs after siRNA treatment for 30 hours. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19563657), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Applications for Human LIN-28A Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and D3 mouse embryonic stem cells

Simple Western

10 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line and JAR human choriocarcinoma cell line

Western Blot

0.1 µg/mL
Sample: JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using AF3757 in the following applications:

Immunocytochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIN-28A

Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1) is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

Long Name

RNA-binding Protein LIN-28

Alternate Names

CSDD1, LIN28, LIN28A, Tex17, ZCCHC1

Entrez Gene IDs

79727 (Human); 83557 (Mouse); 298542 (Rat)

Gene Symbol

LIN28A

UniProt

Q9H9Z2

Additional LIN-28A Products

Product Documents for Human LIN-28A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human LIN-28A Antibody

For research use only

Citations
Reviews

Human LIN-28A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3757

Key Product Details

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Cited:

Label

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LIN-28A Antibody

Detection of Human LIN‑28A by Western Blot.

LIN‑28A in BG01V Human Stem Cells.

LIN‑28A in D3 Mouse Stem Cells.

Detection of Human LIN-28A by Simple WesternTM.

Detection of LIN-28A by Western Blot

Detection of LIN-28A by Western Blot

Detection of LIN-28A by Western Blot

Detection of LIN-28A by Western Blot

Applications for Human LIN-28A Antibody

Immunocytochemistry

Simple Western

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Shipping

Stability & Storage

Background: LIN-28A

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional LIN-28A Products

Product Documents for Human LIN-28A Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Human LIN-28A Antibody

Detection of Human LIN-28A by Simple Western^TM.