Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Laminin alpha3/Laminin‑5 in Human Skin.

Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Laminin alpha3/Laminin-5 Antibody

Application

Recommended Usage

Adhesion Blockade

Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF 10A human breast epithelial cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Immunoprecipitation

Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654.

Western Blot

Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB2144 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Immunohistochemistry (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha3, beta3, and gamma2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

References

Colognato, H. et al. (2000) Dev. Dyn. 218:213.
Stahl, S. et al. (1997) J. Cell Sci. 110:55.
Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Entrez Gene IDs

3909 (Human)

Gene Symbol

LAMA3

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Human Laminin alpha3/Laminin-5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Laminin alpha3/Laminin-5 Antibody

For research use only

Citations
Reviews

Human Laminin alpha3/Laminin-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2144

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Laminin alpha3/Laminin-5 Antibody

Laminin alpha3/Laminin-5 in MCF 10A Human Cell Line.