Detects human Kallikrein 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse KLK5 is observed and less than 10% cross-reactivity with recombinant human KLK8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Kallikrein 5 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 5 (Catalog # 1108-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kallikrein 5 (Catalog # 1108-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 5

Human tissue Kallikrein 5 (hK5, also known as stratum corneum tryptic enzyme) is a serine protease of the human tissue Kallikrein gene family (1, 2). The deduced amino acid sequence of human Kallikrein 5 consists of a signal peptide (Met1 to Gly22), a pro region (Val23 to Arg66) and a mature/active enzyme (Ile67 to Ser293). Kallikrein 5 is expressed mainly in skin, breast, brain and testis and to a lesser extent in many other tissues (3). Kallikrein 5 overexpression is an indicator of poor prognosis in ovarian cancer (4). Kallikrein 5 is a candidate physiological activator of Kallikrein 7 in the stratum corneum (5).

References

Brattsand, M. and T. Egelrud (1999) J. Biol. Chem. 274:30033.
Yousef, G.M. et al. (1999) Anticancer Res. 19:2843.
Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
Yousef, G.M. and E.P. Diamandis (1999) J. Biol. Chem. 274:37511.
Kim, H. et al. (2001) Br. J. Cancer 84:643.

Alternate Names

KLK-L2, KLK5, SCTE

Entrez Gene IDs

25818 (Human); 68668 (Mouse); 690603 (Rat)

Gene Symbol

KLK5

UniProt

Q9Y337

Additional Kallikrein 5 Products

Product Documents for Human Kallikrein 5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Kallikrein 5 Antibody

For research use only

Citations
Reviews

Human Kallikrein 5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1108

Key Product Details

Species Reactivity

Validated:

Cited:

Applications